Gfp hepg2 cells

Author: uamk

August undefined, 2024

WebAt 48 hr following transfection of a GFP reporter construct, cells were evaluated via microscopy and flow cytometry. To assess transfection efficiency, cells were first … WebMay 26, 2015 · The expression of yieF was measured in stably transfected cells HepG2-YieF by quantitative RT-PCR and found up-regulated by 3.89-fold upon Cr(VI) induction. In chromate-reducing ability test, HepG2-YieF cells that harbored the reductase showed significantly higher reducing ability of Cr(VI) than HepG2 control cells.

Insulin signaling and glucose metabolism in different hepatoma cell …

WebAn optimized electroporation protocol was developed for transfection of HepG2 cells stably expressing green fluorescent protein. Electric pulses of 600 V/cm yielded the highest … WebHepG2 Cell Line Origin and Characteristics. HepG2 is an immortalized cell line consisting of human liver carcinoma cells, derived from the liver tissue of a 15-year-old Caucasian … staying motivated in recovery

A polysaccharide from Grifola frondosa relieves insulin ... - PubMed

WebHep G2 cells are passaged 24 hr prior to transfection to give a cell density of 50-60% confluence at the time of transfection. The adherent cells are washed in 1 x HBS, and … WebJan 1, 2014 · To test this hypothesis, each of these CPPs was incubated with an AAV2 vector encoding green fluorescent protein (GFP), and GFP expression was assayed after infection of HEK293T cells. ... Transduction titers of AAV2 in the presence or in the absence of LAH4 in HEK293T cells and HepG2 cells. (f) GFP expression levels of HEK293T … WebApr 12, 2024 · CES2 inhibition in a human liver cell line (HepG2) phenocopies Ces2a deficiency. ... 609 bp and WT Ces2a: 1030 bp. For adenoviral overexpression of CES2 or GFP, mice were fed HFD for 20 weeks and then injected with 1.6 x 109 PFU particles of adenoviral vectors (Vector Biolabs) into the tail vein [8]. Six days post-injection, a … staying kingdom minded womens daily devotion

How can I make HepG2 stable cell line using G418?

GFP Imaging in Fixed Cells BioTechniques

WebHCT 116. CCL-247 ™. HCT 116 cell line was isolated from the colon of an adult male with colon cancer. It has a mutation in codon 13 of the ras proto-oncogene and can be used as a positive control for PCR assays of mutation in this codon. Product category. WebJan 27, 2015 · To make stably NTCP overexpressed HepG2, I used G418 selection vector, pcDNA3.1 (+). Three days after transfection NTCP-FLAG-pcDNA3.1 (+) to HepG2, I conducted immunofluorescence and I confirmed ... staying motivated during the holidaysWebApr 14, 2024 · B HepG2 cells were transfected for 24 h with the indicated combinations of Flag control, Flag-SESN2, Flag-SESN2 and Flag-IGF2BP3-GFP vectors and further cultured in the absence of glucose (Glc ... staying inside disney world resort

"WebAug 28, 2024 · Thereafter culture medium was exchanged and HepG2 or HSC Col-GFP cells were either left for a further 24 h and the supernatant was used for co-immunoprecipitation (HepG2, Section 2.12) or after 10 h medium was exchanged and cells were starved for 16 h and stimulated the next day (HepG2, HSC Col-GFP, Section 2.5). " - Gfp hepg2 cells

Gfp hepg2 cells

Cell-Based Assays Reporter Cells from InvivoGen

WebHepG2-preS1-GFP cells were cultured in R10 culture medium as follows. 1. Prepare R10 medium by adding to RPMI 1640 10% of heat inactivated fetal bovine serum, 20 mM HEPES, 1 mM sodium pyruvate, 100 IU/mL penicillin, 100 μg/mL streptomycin, MeM amino acids, Glutamax, MeM non-essential amino acids, 5 μg/mL Plasmocin. 2. WebIn this study, the hypoglycemic activity of a polysaccharide obtained from Grifola frondosa (GFP) on HepG2 cell and hpyerglycemic mechanism were investigated. The purity of the isolated polysaccharides was examined by HPLC. In this research, it was found that GFP enhanced the absorption of glucose of HepG2 cells in a dose dependent manner at 24 ...

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WebMar 25, 2024 · g, Live-cell imaging of HEPG2 cells that were incubated at 37 °C for 1 h with 50 nM rabbit IgG-647 and 25 nM goat anti-rabbit, goat anti-rabbit M6Pn or goat anti-rabbit GalNAc. Scale bar, 20 µm ... WebJul 5, 2024 · E GFP expression in HepG2-NTCP cells transfected with GFP-encoding plasmids under the control of either a CMV-IE promoter or an EF1α promoter and subsequently cultured in indicated medium for indicated times. Scale bar …

WebEach reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines. Figure 2. WebInvivoGen offers a large panel of cell lines engineered to provide simple, rapid, and reliable monitoring of innate immunity-specific signaling pathways. Our cells stably express one or two reporter proteins, the secreted embryonic alkaline phosphatase (SEAP) and/or the Lucia luciferase. Both systems offer the great advantage of being secreted ...

WebApr 3, 2024 · A GFP fluorescence is eliminated when the proteins are exposed to acetone and GFP is very sensitive to dehydration as well. I suggest that you fix the cells in formaldehyde. I have used formalin and glutaraldehyde successfully in the past. Be certain that your cells don't get dehydrated since that will entirely eliminate the fluorescent signal. WebHepG2, HepG2/GFP and HepG2/GFP-HBx cells were treated with adriamycin (2.5 microg/ml), and apoptosis of the cells was determined by their morphological changes, …

WebJul 21, 2024 · HepG2 cells (from a human hepatoma) were cultured at 37 °C, ... 50 and 100 MOI of the GFP adenovirus and 24 h post-infection the number of GFP positive cells was evaluated. Primary hepatocytes ...

WebJul 10, 2024 · ( A) Generation scheme of a HepG2-derived cell line stably expressing HDV (HepG2-HDV) and HuH7 cells expressing HDV (HuH7-HDV), HDV and the HBV … staying married but living separate livesWebThe influence of GFP-actin expression on the adhesion dynamics of HepG2 cells on a model extracellular matrix Integrins belong to a family of important cell surface receptors which mediate the adhesion of most anchorage-dependent cells to nature extracellular matrix (ECM) and biomaterials. staying motivated about fitnessWebJul 8, 2024 · All the BAC-GFP HepG2 reporter cell lines (p53-GFP, MDM2-GFP, and p21-GFP) were previously established and characterized . The cells were used between passage 14 and 20 and seeded in Greiner black μ-clear 384 well plates, at 8000 cells per well. Immunostaining. Western blot. staying motivated to exerciseWebDec 1, 2024 · Human HepG2 cells, established from a differentiated hepatoma, provide a widely used model system for the study of cell polarization ( 2 ). To understand the mechanism for hepatocyte polarity establishment, we used HepG2 cells in 3D culture with ECM-rich Matrigel. staying married for the sake of the kidsWebApr 19, 2024 · HepG2 cells were infected with the GFP-LC3 adenovirus. After 24 h, the cells were starved in Earle’s balanced salt solution (EBSS, Thermo Fisher Scientific, Waltham, MA, USA, 14155063) for 4 h and treated with ZZY-19 (10 μM), sorafenib (10 μM), or ZZY-19 plus sorafenib for 24 h. staying married but separatedWebHepG2 cells are adherent, epithelial-like cells growing as monolayers and also in small aggregates. About turboGFP protein: tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda … staying motivated to reach goals staying motivated in college