Cisplatin hplc method
WebJul 13, 2016 · The HPLC method was successfully applied to in vitro studies and enabled us to quantify CDDP not only in the NaCl medium but also in the cellular lysates using only 0.02 mL of a sample. Our results clearly demonstrate that the intracellular CDDP concentrations in EHD1-overexpressing cells were lower than those in control cells. WebJun 12, 2000 · In water, the antineoplastic drug cisplatin, cis-[PtCl2(NH3)2] (1) hydrolyses slowly to the aqua complexes cis-[Pt(NH 3) 2 Cl(H 2 O)] + (2) and, to a small extent, cis-[Pt(NH 3) 2 (H 2 O) 2] 2+ (3), which are thought to play an important role in the metabolism of cisplatin.HPLC is a useful technique for monitoring 2 and 3, but only if the …
Cisplatin hplc method
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WebCisplatin hydrochloride is an important chemotherapeutic drug for cancer treatment. It has a low molar absorptivity in the UV region and has no fluorescence. Therefore, a selective … WebSep 1, 2016 · An isocratic reversed-phase high-performance liquid chromatographic method with ultraviolet detection at 254 nm has been developed for the determination of doxorubicin in human plasma. Plasma...
WebMar 1, 2024 · Cisplatin is a platinum containing compound used for the treatment of different types of cancers. Quantitative determination of cisplatin has been carried out … Webderivitized cisplatin [21]. The objective of study was to develop and validate a direct and effective HPLC method for the simultaneous determination of etoposide and cisplatin which could be used in quality control analysis. The present work therefore is a direct method with good linearity and sensitivity for both drugs. O O O H HO H HO O O O O ...
WebCisplatin is an anti-tumor agent widely employed in cancer chemotherapy. A specific and selective method for the quantitative determination of cisplatin in human plasma and its … WebWe successfully determined cisplatin, hydroxo complexes, and OH-dimer by HPLC using a πNAP column. Thus the measurement of cisplatin (cis-diamminedichloro-platinum(II), cis-[PtCl2(NH3)2]) (CDDP) should be done using a πNAP column rather than a C18 column or aminopropylsilyl silica gel column.
WebFor cis-diammine (1,1-cyclobutane-dicarboxylato) platinum (CBDCA), normal-phase chromatography on silica with UV detection suffices down to levels of 10 μM in urine or plasma ultrafiltrates. Quantitation below 10 μM needs HPLC fraction collection followed by flameless atomic absorption spectrophotometry (FAAS; 0.5 μM detectable).
WebA method of preparation and physical properties including the pK a of several species of cisplatin in water,2 the UV and visible spectra, HPLC methods, and mass spectra have been reported.1 Cisplatin is a platinum-containing, broad activity antineoplastic and alkylating agent effective against solid tumors of testes, ovaries, and bladder ... philips alice oneWebA high-performance liquid chromatographic (HPLC) method is described for the determination of residual levels of cisplatin from extracts of surfaces with very low … philips alkco lightingWebTotal and free cisplatin concentrations were measured using a validated HPLC-UV method. Results: The highest concentration of total and free cisplatin was achieved at the end of the infusion in both regimens. Total cisplatin concentration declined 30 minutes after the end of infusion in both the groups. After 1hour of discontinuing cisplatin ... philips all in one cooker 3000 seriesWebIn this work, a new sensitive and selective method of determining cisplatin-DNA adducts based on the use of element-selective mass spectrometry is proposed, and the method is then applied to detect cisplatin adducts induced … philips all in one cooker instructionsWebCisplatin (NSC 119875, Cisplatinum, cis-diamminedichloroplatinum II, CDDP, cis DDP, DDP) is an inorganic platinum complex, which is able to inhibit DNA synthesis by conforming DNA adducts in tumor cells. Cisplatin activates ferroptosis and induces autophagy. Solutions are best fresh-prepared. trustofinWebMethods: A microemulsion method was employed to generate lipid coated cisplatin/oleanolic acid calcium carbonate nanoparticles (CDDP/OA-LCC NPs), and the loading concentration of CDDP and OA was measured by atomic absorption spectroscopy and HPLC respectively.Transmission electron microscopy (TEM) was used to examine … philips all in one cooker recipe book pdfWebHypoxia can induce chemoresistance, which is a significant clinical obstacle in cancer therapy. Here, we assessed development of hypoxia-induced chemoresistance (HICR) against free versus polymeric cisplatin micelles in a triple negative breast cancer cell line, MDA-MB-231. We then explored two strategies for the modulation of HICR against … philips all-in-one cooker hd2151