WebMar 15, 2024 · K. brevifasciata was collected from the Sinpyeongcheon Stream under a collection permit issued by the Ministry of Environment in Korea. K. brevifasciata … WebformedwithacDNAsynthesiskit(Biofact,Korea)according tothemanufacturer’sinstructions.Polymerasechainreac-tion(PCR)wasperformedusinggene-specificprimersand SYBR Green kit (Biofact, Korea). Following primers were used;Glyceraldehyde3-phosphatedehydrogenase(GAPDH)
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Genetic variability comparison of cultured Israeli carp
WebThe total RNA was converted to cDNA with a cDNA synthesis kit (BIOFACT, Korea). Further, SYBR Green (BIOFACT, Korea) was used to specify the expression of the intended genes (Beyrami et al. Citation 2024). The genes were amplified as follows: initial denaturation at 95 °C for 5 min (1X), followed by 35 cycles of 95 °C for 30 s, primer ... WebIn biology, a biofact is dead material of a once-living organism. [1] In 1943, the protozoologist Bruno M. Klein of Vienna (1891–1968) coined the term in his article … WebThe cDNA was then used as a template in qRT PCR to study the transcript accumulation of selected genes (Table S1) using a real-time PCR master mix including SYBR green (BioFact, Korea) along with ... fish in 10 gallon tank